InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: LEF1

Summary

InnateDB Protein

IDBP-33531.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

LEF1

Protein Name

lymphoid enhancer-binding factor 1

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000265165

InnateDB Gene

IDBG-33529 (LEF1)

Protein Structure

UniProt Annotation

Function

Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1- independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. {ECO:0000250, ECO:0000269PubMed:11266540, ECO:0000269PubMed:19653274, ECO:0000269PubMed:2010090}.

Subcellular Localization

Nucleus {ECO:0000255PROSITE- ProRule:PRU00267}. Note=Found in nuclear bodies upon PIASG binding. {ECO:0000250}.

Disease Associations

Tissue Specificity

Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines. {ECO:0000269PubMed:19653274}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 68 experimentally validated interaction(s) in this database.
They are also associated with 24 interaction(s) predicted by orthology.

Experimentally validated
Total	68 [view]
Protein-Protein	59 [view]
Protein-DNA	9 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	24 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000977	RNA polymerase II regulatory region sequence-specific DNA binding
GO:0000978	RNA polymerase II core promoter proximal region sequence-specific DNA binding
GO:0001077	RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription
GO:0001228	RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription
GO:0003677	DNA binding
GO:0003682	chromatin binding
GO:0003700	sequence-specific DNA binding transcription factor activity
GO:0003705	RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity
GO:0005515	protein binding
GO:0008013	beta-catenin binding
GO:0008134	transcription factor binding
GO:0008301	DNA binding, bending
GO:0030284	estrogen receptor activity
GO:0030331	estrogen receptor binding
GO:0035326	enhancer binding
GO:0042393	histone binding
GO:0043027	cysteine-type endopeptidase inhibitor activity involved in apoptotic process
GO:0043565	sequence-specific DNA binding
GO:0044212	transcription regulatory region DNA binding
GO:0045295	gamma-catenin binding
GO:0070016	armadillo repeat domain binding
GO:0070742	C2H2 zinc finger domain binding

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0001569	patterning of blood vessels
GO:0001649	osteoblast differentiation
GO:0001755	neural crest cell migration
GO:0001756	somitogenesis
GO:0001837	epithelial to mesenchymal transition
GO:0001944	vasculature development
GO:0002040	sprouting angiogenesis
GO:0006357	regulation of transcription from RNA polymerase II promoter
GO:0006366	transcription from RNA polymerase II promoter
GO:0008284	positive regulation of cell proliferation
GO:0010628	positive regulation of gene expression
GO:0010718	positive regulation of epithelial to mesenchymal transition
GO:0016055	Wnt signaling pathway
GO:0016202	regulation of striated muscle tissue development
GO:0021542	dentate gyrus development
GO:0021766	hippocampus development
GO:0021854	hypothalamus development
GO:0021861	forebrain radial glial cell differentiation
GO:0021873	forebrain neuroblast division
GO:0021879	forebrain neuron differentiation
GO:0021943	formation of radial glial scaffolds
GO:0022407	regulation of cell-cell adhesion
GO:0022408	negative regulation of cell-cell adhesion
GO:0022409	positive regulation of cell-cell adhesion
GO:0030111	regulation of Wnt signaling pathway
GO:0030223	neutrophil differentiation
GO:0030307	positive regulation of cell growth
GO:0030326	embryonic limb morphogenesis
GO:0030335	positive regulation of cell migration
GO:0030509	BMP signaling pathway
GO:0030854	positive regulation of granulocyte differentiation
GO:0030879	mammary gland development
GO:0032696	negative regulation of interleukin-13 production
GO:0032713	negative regulation of interleukin-4 production
GO:0032714	negative regulation of interleukin-5 production
GO:0033153	T cell receptor V(D)J recombination
GO:0042100	B cell proliferation
GO:0042475	odontogenesis of dentin-containing tooth
GO:0043066	negative regulation of apoptotic process
GO:0043154	negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0043392	negative regulation of DNA binding
GO:0043401	steroid hormone mediated signaling pathway
GO:0043586	tongue development
GO:0043923	positive regulation by host of viral transcription
GO:0043966	histone H3 acetylation
GO:0043967	histone H4 acetylation
GO:0045063	T-helper 1 cell differentiation
GO:0045843	negative regulation of striated muscle tissue development
GO:0045892	negative regulation of transcription, DNA-templated
GO:0045893	positive regulation of transcription, DNA-templated
GO:0045944	positive regulation of transcription from RNA polymerase II promoter
GO:0046632	alpha-beta T cell differentiation
GO:0048069	eye pigmentation
GO:0048341	paraxial mesoderm formation
GO:0048468	cell development
GO:0048747	muscle fiber development
GO:0050909	sensory perception of taste
GO:0060021	palate development
GO:0060033	anatomical structure regression
GO:0060070	canonical Wnt signaling pathway
GO:0060325	face morphogenesis
GO:0060326	cell chemotaxis
GO:0060561	apoptotic process involved in morphogenesis
GO:0060710	chorio-allantoic fusion
GO:0061153	trachea gland development
GO:0071345	cellular response to cytokine stimulus
GO:0071353	cellular response to interleukin-4
GO:0071864	positive regulation of cell proliferation in bone marrow
GO:0071866	negative regulation of apoptotic process in bone marrow
GO:0071895	odontoblast differentiation
GO:0071899	negative regulation of estrogen receptor binding
GO:0090068	positive regulation of cell cycle process
GO:0090090	negative regulation of canonical Wnt signaling pathway
GO:1902262	apoptotic process involved in patterning of blood vessels