InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: CYP4F3

Summary

InnateDB Protein

IDBP-34903.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

CYP4F3

Protein Name

cytochrome P450, family 4, subfamily F, polypeptide 3

Synonyms

CPF3; CYP4F; LTB4H;

Species

Homo sapiens

Ensembl Protein

ENSP00000221307

InnateDB Gene

IDBG-34860 (CYP4F3)

Protein Structure

UniProt Annotation

Function

Cytochromes P450 are a group of heme-thiolate monooxygenases. This enzyme requires molecular oxygen and NADPH. Isoform CYP4F3A catalyzes the omega-hydroxylation of LTB4, a potent chemoattractant for polymorphonuclear leukocytes, it has low activity for arachidonic acid. {ECO:0000269PubMed:11461919}.Isoform CYP4F3B: Has a 30-fold higher Km for LTB4 compared with CYP4F3A, but it utilizes arachidonic acid as a substrate for omega-hydroxylation and generates 20-HETE, an activator of kinases PKC and CK2. {ECO:0000269PubMed:11461919}.

Subcellular Localization

Endoplasmic reticulum membrane {ECO:0000250}; Single-pass membrane protein {ECO:0000250}. Microsome membrane {ECO:0000250}; Single-pass membrane protein {ECO:0000250}.

Disease Associations

Tissue Specificity

Isoform CYP4F3A is expressed in the polymorphonuclear leukocytes as well as leukocytes and bone marrow. Isoform CYP4F3B is selectively expressed in liver and kidney and is also the predominant CYP4F isoform in trachea and tissues of the gastrointestinal tract. {ECO:0000269PubMed:11461919}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 2 experimentally validated interaction(s) in this database.

Experimentally validated
Total	2 [view]
Protein-Protein	1 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004497	monooxygenase activity
GO:0005506	iron ion binding
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0020037	heme binding
GO:0050051	leukotriene-B4 20-monooxygenase activity
GO:0052871	alpha-tocopherol omega-hydroxylase activity

Biological Process

GO:0006690	icosanoid metabolic process
GO:0006691	leukotriene metabolic process
GO:0006805	xenobiotic metabolic process
GO:0019369	arachidonic acid metabolic process
GO:0036101	leukotriene B4 catabolic process
GO:0044281	small molecule metabolic process
GO:0055114	oxidation-reduction process

Cellular Component

GO:0005789	endoplasmic reticulum membrane
GO:0016021	integral component of membrane

Protein Structure and Domains

PDB ID

InterPro

IPR001128 Cytochrome P450
IPR002401 Cytochrome P450, E-class, group I
IPR002402 Cytochrome P450, E-class, group II
IPR002403 Cytochrome P450, E-class, group IV

PFAM

PF00067

PRINTS

PR00385
PR00463
PR00464
PR00465

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q08477