InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: RAB8A

Summary

InnateDB Protein

IDBP-35331.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

RAB8A

Protein Name

RAB8A, member RAS oncogene family

Synonyms

MEL; RAB8;

Species

Homo sapiens

Ensembl Protein

ENSP00000300935

InnateDB Gene

IDBG-35329 (RAB8A)

Protein Structure

UniProt Annotation

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Plays an important role in ciliogenesis. Together with MICALL2, may also regulate adherens junction assembly. May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis. {ECO:0000269PubMed:20890297, ECO:0000269PubMed:21282656, ECO:0000269PubMed:21844891}.

Subcellular Localization

Cell membrane {ECO:0000305}; Lipid-anchor {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Golgi apparatus. Recycling endosome membrane. Cell projection, cilium. Cytoplasmic vesicle, phagosome. Cytoplasmic vesicle, phagosome membrane {ECO:0000250}; Lipid-anchor {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Note=Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane. In the GDP- bound form, present in the perinuclear region. Shows a polarized distribution to distal regions of cell protrusions in the GTP- bound form. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical membrane initiation sites (AMIS) during the generation of apical surface and lumenogenesis. Localizes to tubular recycling endosome. Recruited to phagosomes containing S.aureus or M.tuberculosis.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 57 experimentally validated interaction(s) in this database.
They are also associated with 9 interaction(s) predicted by orthology.

Experimentally validated
Total	57 [view]
Protein-Protein	54 [view]
Protein-DNA	3 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	9 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003924	GTPase activity
GO:0005515	protein binding
GO:0005525	GTP binding
GO:0017137	Rab GTPase binding
GO:0019003	GDP binding
GO:0019901	protein kinase binding

Biological Process

GO:0000086	G2/M transition of mitotic cell cycle
GO:0000278	mitotic cell cycle
GO:0006184	GTP catabolic process
GO:0006886	intracellular protein transport
GO:0006904	vesicle docking involved in exocytosis
GO:0006913	nucleocytoplasmic transport
GO:0007165	signal transduction
GO:0007264	small GTPase mediated signal transduction
GO:0007409	axonogenesis
GO:0015031	protein transport
GO:0032869	cellular response to insulin stimulus
GO:0032880	regulation of protein localization
GO:0042384	cilium assembly
GO:0048169	regulation of long-term neuronal synaptic plasticity
GO:0048210	Golgi vesicle fusion to target membrane
GO:0051223	regulation of protein transport
GO:0061024	membrane organization
GO:0072659	protein localization to plasma membrane

Cellular Component

GO:0000139	Golgi membrane
GO:0005622	intracellular
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005794	Golgi apparatus
GO:0005813	centrosome
GO:0005886	plasma membrane
GO:0005929	cilium
GO:0014069	postsynaptic density
GO:0016020	membrane
GO:0016023	cytoplasmic membrane-bounded vesicle
GO:0030140	trans-Golgi network transport vesicle
GO:0030425	dendrite
GO:0030659	cytoplasmic vesicle membrane
GO:0030670	phagocytic vesicle membrane
GO:0031513	nonmotile primary cilium
GO:0036064	ciliary basal body
GO:0043025	neuronal cell body
GO:0045335	phagocytic vesicle
GO:0055037	recycling endosome
GO:0055038	recycling endosome membrane
GO:0070062	extracellular vesicular exosome

Protein Structure and Domains

PDB ID

InterPro

IPR001806 Small GTPase superfamily
IPR002041 Ran GTPase
IPR003578 Small GTPase superfamily, Rho type
IPR003579 Small GTPase superfamily, Rab type
IPR005225 Small GTP-binding protein domain
IPR006689 Small GTPase superfamily, ARF/SAR type
IPR006762 Gtr1/RagA G protein
IPR013684 Mitochondrial Rho-like
IPR020849 Small GTPase superfamily, Ras type
IPR024156 Small GTPase superfamily, ARF type
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM

PF00071
PF00025
PF04670
PF08477

PRINTS

PR00449
PR00627
PR00328

PIRSF

SMART

SM00176
SM00174
SM00175
SM00173
SM00177

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt