InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: PAM

Summary

InnateDB Protein

IDBP-374352.4

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

PAM

Protein Name

peptidylglycine alpha-amidating monooxygenase

Synonyms

PAL; PHM;

Species

Homo sapiens

Ensembl Protein

ENSP00000396493

InnateDB Gene

IDBG-35530 (PAM)

Protein Structure

UniProt Annotation

Function

Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.

Subcellular Localization

Isoform 1: Membrane; Single-pass type I membrane protein.Isoform 2: Membrane; Single-pass type I membrane protein.Isoform 3: Secreted. Note=Secreted from secretory granules.Isoform 4: Secreted. Note=Secreted from secretory granules.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 8 experimentally validated interaction(s) in this database.

Experimentally validated
Total	8 [view]
Protein-Protein	8 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003824	catalytic activity
GO:0004497	monooxygenase activity
GO:0004504	peptidylglycine monooxygenase activity
GO:0004598	peptidylamidoglycolate lyase activity
GO:0005507	copper ion binding
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0016715	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced ascorbate as one donor, and incorporation of one atom of oxygen
GO:0031418	L-ascorbic acid binding

Biological Process

GO:0001519	peptide amidation
GO:0006518	peptide metabolic process
GO:0055114	oxidation-reduction process

Cellular Component

GO:0016020	membrane
GO:0016021	integral component of membrane
GO:0070062	extracellular vesicular exosome

Protein Structure and Domains

PDB ID

InterPro

IPR000323 Copper type II, ascorbate-dependent monooxygenase, N-terminal
IPR000720 Peptidyl-glycine alpha-amidating monooxygenase
IPR001258 NHL repeat
IPR008977 PHM/PNGase F domain
IPR013017 NHL repeat, subgroup

PFAM

PF01082
PF01436

PRINTS

PR00790

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

P19021