InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: HUS1

Summary

InnateDB Protein

IDBP-374856.4

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

HUS1

Protein Name

HUS1 checkpoint homolog (S. pombe)

Synonyms

hHUS1;

Species

Homo sapiens

Ensembl Protein

ENSP00000403844

InnateDB Gene

IDBG-16063 (HUS1)

Protein Structure

UniProt Annotation

Function

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. {ECO:0000269PubMed:21659603}.

Subcellular Localization

Nucleus. Cytoplasm. Note=In discrete nuclear foci upon DNA damage. According to PubMed:14500360, localized also in the cytoplasm. DNA damage induces its nuclear translocation. Shuttles between the nucleus and the cytoplasm.

Disease Associations

Tissue Specificity

Ubiquitous. {ECO:0000269PubMed:10777662}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 38 experimentally validated interaction(s) in this database.

Experimentally validated
Total	38 [view]
Protein-Protein	36 [view]
Protein-DNA	2 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005515	protein binding

Biological Process

GO:0000077	DNA damage checkpoint
GO:0006260	DNA replication
GO:0006281	DNA repair
GO:0006974	cellular response to DNA damage stimulus
GO:0071479	cellular response to ionizing radiation