InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: TRIM32

Summary

InnateDB Protein

IDBP-378852.4

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

TRIM32

Protein Name

tripartite motif containing 32

Synonyms

BBS11; HT2A; LGMD2H; TATIP;

Species

Homo sapiens

Ensembl Protein

ENSP00000408292

InnateDB Gene

IDBG-82716 (TRIM32)

Protein Structure

UniProt Annotation

Function

Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May ubiquitinate BBS2. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo. {ECO:0000269PubMed:19349376, ECO:0000269PubMed:22500027}.

Subcellular Localization

Cytoplasm. Note=Localized in cytoplasmic bodies, often located around the nucleus.

Disease Associations

Limb-girdle muscular dystrophy 2H (LGMD2H) [MIM:254110]: An autosomal recessive degenerative myopathy characterized by pelvic girdle, shoulder girdle and quadriceps muscle weakness. Clinical phenotype and severity are highly variable. Disease progression is slow and most patients remain ambulatory into the sixth decade of life. {ECO:0000269PubMed:11822024, ECO:0000269PubMed:17994549}. Note=The disease is caused by mutations affecting the gene represented in this entry.Bardet-Biedl syndrome 11 (BBS11) [MIM:209900]: A syndrome characterized by usually severe pigmentary retinopathy, early- onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. Bardet-Biedl syndrome inheritance is autosomal recessive, but three mutated alleles (two at one locus, and a third at a second locus) may be required for clinical manifestation of some forms of the disease. {ECO:0000269PubMed:16606853}. Note=The disease is caused by mutations affecting the gene represented in this entry. It has been suggested that TRIM32 might be the E3 ubiquitin ligase for BBS2, a component of the BBSome complex involved in ciliogenesis, that is ubiquitinated and degraded by the proteasome (PubMed:22500027). {ECO:0000269PubMed:22500027}.

Tissue Specificity

Spleen, thymus, prostate, testis, ovary, intestine, colon and skeletal muscle. {ECO:0000269PubMed:11822024}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 77 experimentally validated interaction(s) in this database.
They are also associated with 7 interaction(s) predicted by orthology.

Experimentally validated
Total	77 [view]
Protein-Protein	77 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	7 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003713	transcription coactivator activity
GO:0003723	RNA binding
GO:0004842	ubiquitin-protein transferase activity
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0016874	ligase activity
GO:0017022	myosin binding
GO:0030957	Tat protein binding
GO:0031369	translation initiation factor binding
GO:0043130	ubiquitin binding
GO:0043621	protein self-association
GO:0046872	metal ion binding

Biological Process

GO:0000209	protein polyubiquitination
GO:0009411	response to UV
GO:0016567	protein ubiquitination
GO:0030307	positive regulation of cell growth
GO:0030335	positive regulation of cell migration
GO:0032479	regulation of type I interferon production
GO:0032481	positive regulation of type I interferon production
GO:0032897	negative regulation of viral transcription
GO:0034612	response to tumor necrosis factor
GO:0042787	protein ubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0043123	positive regulation of I-kappaB kinase/NF-kappaB signaling
GO:0045087	innate immune response (InnateDB)
GO:0045444	fat cell differentiation
GO:0045666	positive regulation of neuron differentiation
GO:0045732	positive regulation of protein catabolic process
GO:0045787	positive regulation of cell cycle
GO:0045862	positive regulation of proteolysis
GO:0048147	negative regulation of fibroblast proliferation
GO:0050769	positive regulation of neurogenesis
GO:0051091	positive regulation of sequence-specific DNA binding transcription factor activity
GO:0051092	positive regulation of NF-kappaB transcription factor activity
GO:1902187	negative regulation of viral release from host cell
GO:1902230	negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage
GO:2000147	positive regulation of cell motility

Cellular Component

GO:0005622	intracellular
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0005863	striated muscle myosin thick filament

Protein Structure and Domains

PDB ID

InterPro

IPR000315 Zinc finger, B-box
IPR001258 NHL repeat
IPR001841 Zinc finger, RING-type
IPR013017 NHL repeat, subgroup
IPR018957 Zinc finger, C3HC4 RING-type

PFAM

PF00643
PF01436
PF13639
PF14634
PF00097

PRINTS

PIRSF

SMART

SM00336
SM00184

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q13049