Homo sapiens Protein: ECT2
InnateDB Protein IDBP-385484.5
Last Modified 2014-10-13 [Report errors or provide feedback]
Gene Symbol ECT2
Protein Name epithelial cell transforming sequence 2 oncogene
Synonyms ARHGEF31;
Species Homo sapiens
Ensembl Protein ENSP00000415876
InnateDB Gene IDBG-65239 (ECT2)
Protein Structure
UniProt Annotation
Function Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)- induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death. {ECO:0000269PubMed:10579713, ECO:0000269PubMed:14645260, ECO:0000269PubMed:15254234, ECO:0000269PubMed:15545273, ECO:0000269PubMed:15642749, ECO:0000269PubMed:16103226, ECO:0000269PubMed:16170345, ECO:0000269PubMed:16236794, ECO:0000269PubMed:16495035, ECO:0000269PubMed:19129481, ECO:0000269PubMed:19468300, ECO:0000269PubMed:19617897, ECO:0000269PubMed:21189248, ECO:0000269PubMed:21373644}.
Subcellular Localization Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Cleavage furrow. Midbody. Cell junction. Cell junction, tight junction. Note=Sequestered within the nucleus during interphase. Dispersed throughout the cytoplasm upon breakdown of the nuclear envelope during mitosis. Colocalizes with the centralspindlin complex to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalized with RhoA at the midbody. Its subcellular localization to tight junction is increased by calcium. Localized predominantly in the cytoplasm of numerous carcinoma cells.
Disease Associations
Tissue Specificity Expressed in lung epithelial cells (at protein level). Expressed in squamous cell carcinoma, primary non-small cell lung cancer tumors and lung adenocarcinoma. {ECO:0000269PubMed:19617897}.
Number of Interactions This gene and/or its encoded proteins are associated with 151 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.
Experimentally validated
Total 151 [view]
Protein-Protein 151 [view]
Protein-DNA 0
Protein-RNA 0
Predicted by orthology
Total 1 [view]
Gene Ontology

Molecular Function
Accession GO Term
GO:0004871 signal transducer activity
GO:0005085 guanyl-nucleotide exchange factor activity
GO:0005089 Rho guanyl-nucleotide exchange factor activity
GO:0005096 GTPase activator activity
GO:0005515 protein binding
GO:0042803 protein homodimerization activity
Biological Process
GO:0000902 cell morphogenesis
GO:0000910 cytokinesis
GO:0007264 small GTPase mediated signal transduction
GO:0015031 protein transport
GO:0032147 activation of protein kinase activity
GO:0032321 positive regulation of Rho GTPase activity
GO:0032467 positive regulation of cytokinesis
GO:0032862 activation of Rho GTPase activity
GO:0032863 activation of Rac GTPase activity
GO:0035023 regulation of Rho protein signal transduction
GO:0042307 positive regulation of protein import into nucleus
GO:0043065 positive regulation of apoptotic process
GO:0043089 positive regulation of Cdc42 GTPase activity
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB signaling
GO:0043547 positive regulation of GTPase activity
GO:0045666 positive regulation of neuron differentiation
GO:0045859 regulation of protein kinase activity
GO:0048011 neurotrophin TRK receptor signaling pathway
GO:0051056 regulation of small GTPase mediated signal transduction
GO:0051260 protein homooligomerization
GO:0051988 regulation of attachment of spindle microtubules to kinetochore
GO:0070301 cellular response to hydrogen peroxide
GO:0070830 tight junction assembly
GO:0071277 cellular response to calcium ion
GO:0071479 cellular response to ionizing radiation
GO:0097190 apoptotic signaling pathway
Cellular Component
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005911 cell-cell junction
GO:0005923 tight junction
GO:0030496 midbody
GO:0032154 cleavage furrow
GO:0072686 mitotic spindle
GO:0097149 centralspindlin complex
Protein Structure and Domains
InterPro IPR000219 Dbl homology (DH) domain
IPR001357 BRCT domain
PFAM PF00621
Post-translational Modifications
SwissProt Q9H8V3
PhosphoSite PhosphoSite-Q9H8V3
UniProt Splice Variant
Entrez Gene 1894
UniGene Hs.741516
OMIM 600586
HPRD 11860
EMBL AC108667 AK001323 AK023267 AK027713 AK314581 AL137710 AY376439 BC006838 BC112086 DQ847274
GenPept AAH06838 AAI12087 AAQ83675 ABH10140 BAA91624 BAB14498 BAB55317 BAG37157 CAB70886