InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: VPS4A

Summary

InnateDB Protein

IDBP-39002.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

VPS4A

Protein Name

vacuolar protein sorting 4 homolog A (S. cerevisiae)

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000254950

InnateDB Gene

IDBG-39000 (VPS4A)

Protein Structure

UniProt Annotation

Function

Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission (PubMed:24814515). {ECO:0000269PubMed:11563910, ECO:0000269PubMed:11595185, ECO:0000269PubMed:15075231, ECO:0000269PubMed:24814515}.

Subcellular Localization

Prevacuolar compartment membrane; Peripheral membrane protein. Late endosome membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}. Midbody. Note=Membrane-associated in the prevacuolar endosomal compartment. Localizes to the midbody of dividing cells, interaction with ZFYVE19/ANCHR mediates retention at midbody (PubMed:24814515). Localized in two distinct rings on either side of the Fleming body. {ECO:0000269PubMed:24814515}.

Disease Associations

Tissue Specificity

Ubiquitously expressed. {ECO:0000269PubMed:10637304, ECO:0000269PubMed:11563910}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 25 experimentally validated interaction(s) in this database.

Experimentally validated
Total	25 [view]
Protein-Protein	25 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0008022	protein C-terminus binding
GO:0009378	four-way junction helicase activity
GO:0016887	ATPase activity
GO:0019904	protein domain specific binding
GO:0042623	ATPase activity, coupled

Biological Process

GO:0000910	cytokinesis
GO:0006200	ATP catabolic process
GO:0006281	DNA repair
GO:0006310	DNA recombination
GO:0009838	abscission
GO:0015031	protein transport
GO:0016032	viral process
GO:0016192	vesicle-mediated transport
GO:0016197	endosomal transport
GO:0019058	viral life cycle
GO:0031565	cytokinesis checkpoint
GO:0032466	negative regulation of cytokinesis
GO:0061024	membrane organization

Cellular Component

GO:0000815	ESCRT III complex
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0030496	midbody
GO:0031902	late endosome membrane
GO:0048471	perinuclear region of cytoplasm
GO:0070062	extracellular vesicular exosome

Protein Structure and Domains

PDB ID

InterPro

IPR002611 IstB-like ATP-binding protein
IPR003593 AAA+ ATPase domain
IPR003959 ATPase, AAA-type, core
IPR007330 MIT
IPR008824 DNA helicase, Holliday junction RuvB type, N-terminal
IPR011704 ATPase, dynein-related, AAA domain
IPR015415 Vps4 oligomerisation, C-terminal
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM