Homo sapiens Protein: RIPK3
InnateDB Protein IDBP-3904.6
Last Modified 2014-10-13 [Report errors or provide feedback]
Gene Symbol RIPK3
Protein Name receptor-interacting serine-threonine kinase 3
Synonyms RIP3;
Species Homo sapiens
Ensembl Protein ENSP00000216274
InnateDB Gene IDBG-3902 (RIPK3)
Protein Structure
UniProt Annotation
Function Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production. {ECO:0000269PubMed:19498109, ECO:0000269PubMed:19524512, ECO:0000269PubMed:19524513, ECO:0000269PubMed:22265413, ECO:0000269PubMed:22265414, ECO:0000269PubMed:22421439}.
Subcellular Localization Cytoplasm, cytosol {ECO:0000269PubMed:22265413}. Cell membrane {ECO:0000250}. Mitochondrion {ECO:0000305}.
Disease Associations
Tissue Specificity Highly expressed in the pancreas. Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers. {ECO:0000269PubMed:15896315}.
Number of Interactions This gene and/or its encoded proteins are associated with 28 experimentally validated interaction(s) in this database.
They are also associated with 2 interaction(s) predicted by orthology.
Experimentally validated
Total 28 [view]
Protein-Protein 28 [view]
Protein-DNA 0
Protein-RNA 0
Predicted by orthology
Total 2 [view]
Gene Ontology

Molecular Function
Accession GO Term
GO:0003713 transcription coactivator activity
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0004704 NF-kappaB-inducing kinase activity
GO:0004713 protein tyrosine kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016772 transferase activity, transferring phosphorus-containing groups
GO:0032403 protein complex binding
GO:0042802 identical protein binding
Biological Process
GO:0001914 regulation of T cell mediated cytotoxicity
GO:0002224 toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0002819 regulation of adaptive immune response
GO:0006464 cellular protein modification process
GO:0006468 protein phosphorylation
GO:0007165 signal transduction
GO:0007249 I-kappaB kinase/NF-kappaB signaling
GO:0010922 positive regulation of phosphatase activity
GO:0032147 activation of protein kinase activity
GO:0032481 positive regulation of type I interferon production
GO:0032649 regulation of interferon-gamma production
GO:0033077 T cell differentiation in thymus
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0035666 TRIF-dependent toll-like receptor signaling pathway
GO:0038061 NIK/NF-kappaB signaling
GO:0043029 T cell homeostasis
GO:0045087 innate immune response (InnateDB)
GO:0046006 regulation of activated T cell proliferation
GO:0046777 protein autophosphorylation
GO:0048535 lymph node development
GO:0048536 spleen development
GO:0048538 thymus development
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051260 protein homooligomerization
GO:0051291 protein heterooligomerization
GO:0051347 positive regulation of transferase activity
GO:0051351 positive regulation of ligase activity
GO:0051353 positive regulation of oxidoreductase activity
GO:0060545 positive regulation of necroptotic process
GO:0070235 regulation of activation-induced cell death of T cells
GO:0070266 necroptotic process
GO:0090312 positive regulation of protein deacetylation
GO:0097190 apoptotic signaling pathway
GO:1990000 amyloid fibril formation
GO:2000377 regulation of reactive oxygen species metabolic process
GO:2000379 positive regulation of reactive oxygen species metabolic process
GO:2000452 regulation of CD8-positive, alpha-beta cytotoxic T cell extravasation
GO:2001244 positive regulation of intrinsic apoptotic signaling pathway
Cellular Component
GO:0005622 intracellular
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0097342 ripoptosome
Protein Structure and Domains
InterPro IPR000719 Protein kinase domain
IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain
IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain
IPR011009 Protein kinase-like domain
IPR020635 Tyrosine-protein kinase, catalytic domain
PFAM PF00069
Post-translational Modifications
SwissProt Q9Y572
PhosphoSite PhosphoSite-Q9Y572
UniProt Splice Variant
Entrez Gene 11035
UniGene Hs.268551
RefSeq NP_006862
OMIM 605817
HPRD 09315
EMBL AF156884 AK296140 AL096870 AY453693 AY494982 AY494983 BC062584 CH471078
GenPept AAD39005 AAH62584 AAS16359 AAS75516 AAS75517 BAG58881 EAW66021