InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Ogt

Summary

InnateDB Protein

IDBP-400663.4

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Ogt

Protein Name

O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)

Synonyms

1110038P24Rik; 4831420N21Rik; AI115525; Ogtl;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000113454

InnateDB Gene

IDBG-164680 (Ogt)

Protein Structure

UniProt Annotation

Function

Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta- linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. Plays a key role in chromatin structure by mediating O- GlcNAcylation of 'Ser-112' of histone H2B: recruited to CpG-rich transcription start sites of active genes via its interaction with TET proteins (TET1, TET2 or TET3). As part of the NSL complex indirectly involved in acetylation of nucleosomal histone H4 on several lysine residues. O-GlcNAcylation of 'Ser-75' of EZH2 increases its stability, and facilitating the formation of H3K27me3 by the PRC2/EED-EZH2 complex (By similarity). Regulates circadian oscillation of the clock genes and glucose homeostasis in the liver. Stabilizes clock proteins ARNTL/BMAL1 and CLOCK through O-glycosylation, which prevents their ubiquitination and subsequent degradation. Promotes the CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2 and CRY1/2. {ECO:0000250, ECO:0000269PubMed:23337503, ECO:0000269PubMed:23395176}.

Subcellular Localization

Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}. Cell membrane {ECO:0000250}. Note=Mostly in the nucleus. Retained in the nucleus via interaction with HCFC1. After insulin induction, translocated from the nucleus to the cell membrane via phophatidylinisotide binding. Colocalizes with AKT1 at the plasma membrane (By similarity). {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 24 experimentally validated interaction(s) in this database.
They are also associated with 33 interaction(s) predicted by orthology.

Experimentally validated
Total	24 [view]
Protein-Protein	24 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	33 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003824	catalytic activity
GO:0005515	protein binding
GO:0005547	phosphatidylinositol-3,4,5-trisphosphate binding
GO:0008047	enzyme activator activity
GO:0008080	N-acetyltransferase activity
GO:0016262	protein N-acetylglucosaminyltransferase activity
GO:0043995	histone acetyltransferase activity (H4-K5 specific)
GO:0043996	histone acetyltransferase activity (H4-K8 specific)
GO:0046972	histone acetyltransferase activity (H4-K16 specific)
GO:0097363	protein O-GlcNAc transferase activity

Biological Process

GO:0006110	regulation of glycolytic process
GO:0006493	protein O-linked glycosylation
GO:0006915	apoptotic process
GO:0030854	positive regulation of granulocyte differentiation
GO:0031397	negative regulation of protein ubiquitination
GO:0032868	response to insulin
GO:0032922	circadian regulation of gene expression
GO:0035020	regulation of Rac protein signal transduction
GO:0043085	positive regulation of catalytic activity
GO:0043981	histone H4-K5 acetylation
GO:0043982	histone H4-K8 acetylation
GO:0043984	histone H4-K16 acetylation
GO:0045862	positive regulation of proteolysis
GO:0045944	positive regulation of transcription from RNA polymerase II promoter
GO:0046626	regulation of insulin receptor signaling pathway
GO:0048015	phosphatidylinositol-mediated signaling
GO:0051571	positive regulation of histone H3-K4 methylation
GO:0061087	positive regulation of histone H3-K27 methylation
GO:0071300	cellular response to retinoic acid
GO:0080182	histone H3-K4 trimethylation
GO:0090526	regulation of gluconeogenesis involved in cellular glucose homeostasis

Cellular Component

GO:0000123	histone acetyltransferase complex
GO:0005622	intracellular
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005813	centrosome
GO:0005829	cytosol
GO:0005886	plasma membrane
GO:0070688	MLL5-L complex

Protein Structure and Domains

PDB ID

MGI:1339639

InterPro

IPR001440 Tetratricopeptide TPR1
IPR013026 Tetratricopeptide repeat-containing domain
IPR013105 Tetratricopeptide TPR2
IPR019734 Tetratricopeptide repeat

PFAM

PF00515
PF07719
PF13174
PF13176
PF13181

PRINTS

PIRSF

SMART

SM00028

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q8CGY8