InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: NEIL3

Summary

InnateDB Protein

IDBP-45524.5

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

NEIL3

Protein Name

nei endonuclease VIII-like 3 (E. coli)

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000264596

InnateDB Gene

IDBG-45522 (NEIL3)

Protein Structure

UniProt Annotation

Function

DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5- OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair. {ECO:0000269PubMed:12433996, ECO:0000269PubMed:19170771, ECO:0000269PubMed:22569481, ECO:0000269PubMed:23755964}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:12433996}.

Disease Associations

Tissue Specificity

Expressed in keratinocytes and embryonic fibroblasts (at protein level). Also detected in thymus, testis and fetal lung primary fibroblasts. {ECO:0000269PubMed:12433996, ECO:0000269PubMed:16428305, ECO:0000269PubMed:19426544, ECO:0000269PubMed:22365498}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 3 experimentally validated interaction(s) in this database.

Experimentally validated
Total	3 [view]
Protein-Protein	1 [view]
Protein-DNA	2 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000405	bubble DNA binding
GO:0003676	nucleic acid binding
GO:0003684	damaged DNA binding
GO:0003690	double-stranded DNA binding
GO:0003697	single-stranded DNA binding
GO:0003906	DNA-(apurinic or apyrimidinic site) lyase activity
GO:0008270	zinc ion binding
GO:0016799	hydrolase activity, hydrolyzing N-glycosyl compounds
GO:0019104	DNA N-glycosylase activity

Biological Process

GO:0000737	DNA catabolic process, endonucleolytic
GO:0006281	DNA repair
GO:0006284	base-excision repair
GO:0006289	nucleotide-excision repair

Cellular Component

GO:0005634

nucleus

Protein Structure and Domains

PDB ID

InterPro

IPR000214 Zinc finger, DNA glycosylase/AP lyase-type
IPR001876 Zinc finger, RanBP2-type
IPR010666 Zinc finger, GRF-type
IPR010979 Ribosomal protein S13-like, H2TH
IPR012319 DNA glycosylase/AP lyase, catalytic domain
IPR015886 DNA glycosylase/AP lyase, H2TH DNA-binding

PFAM

PF00641
PF06839
PF01149
PF06831

PRINTS

PIRSF

SMART

SM00547
SM00898

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q8TAT5