InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: TUT1

Summary

InnateDB Protein

IDBP-485555.3

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

TUT1

Protein Name

terminal uridylyl transferase 1, U6 snRNA-specific

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000419607

InnateDB Gene

IDBG-51191 (TUT1)

Protein Structure

UniProt Annotation

Function

Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication- dependent histone mRNA degradation. {ECO:0000269PubMed:16790842, ECO:0000269PubMed:18288197, ECO:0000269PubMed:21102410}.

Subcellular Localization

Nucleus, nucleolus. Nucleus speckle.

Disease Associations

Tissue Specificity

Widely expressed. {ECO:0000269PubMed:18288197}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 30 experimentally validated interaction(s) in this database.

Experimentally validated
Total	30 [view]
Protein-Protein	29 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003676	nucleic acid binding
GO:0003723	RNA binding
GO:0003730	mRNA 3'-UTR binding
GO:0004652	polynucleotide adenylyltransferase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0019899	enzyme binding
GO:0046872	metal ion binding
GO:0050265	RNA uridylyltransferase activity

Biological Process

GO:0006378	mRNA polyadenylation
GO:0006379	mRNA cleavage
GO:0016180	snRNA processing

Cellular Component

GO:0005634	nucleus
GO:0005730	nucleolus
GO:0005847	mRNA cleavage and polyadenylation specificity factor complex
GO:0016607	nuclear speck
GO:0045171	intercellular bridge