InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: MCM8

Summary

InnateDB Protein

IDBP-51963.7

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

MCM8

Protein Name

minichromosome maintenance complex component 8

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000265187

InnateDB Gene

IDBG-51957 (MCM8)

Protein Structure

UniProt Annotation

Function

Component of the MCM8-MCM9 complex, a complex involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. The MCM8- MCM9 complex probably acts as a hexameric helicase downstream of the Fanconi anemia proteins BRCA2 and RAD51 and is required to process aberrant forks into homologous recombination substrates and to orchestrate homologous recombination with resection, fork stabilization and fork restart. May also play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition complex (ORC). Binds chromatin throughout the cell cycle. {ECO:0000269PubMed:22771115}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:12527764, ECO:0000269PubMed:18072282}. Note=Localizes to nuclear foci and colocalizes with RAD51.

Disease Associations

Tissue Specificity

Highest levels in placenta, lung and pancreas. Low levels in skeletal muscle and kidney. Expressed in various tumors with highest levels in colon and lung cancers. {ECO:0000269PubMed:12771218}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 13 experimentally validated interaction(s) in this database.

Experimentally validated
Total	13 [view]
Protein-Protein	9 [view]
Protein-DNA	3 [view]
Protein-RNA	0
DNA-DNA	1 [view]
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0004386	helicase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016851	magnesium chelatase activity
GO:0016887	ATPase activity

Biological Process

GO:0000082	G1/S transition of mitotic cell cycle
GO:0000278	mitotic cell cycle
GO:0006260	DNA replication
GO:0006271	DNA strand elongation involved in DNA replication
GO:0006974	cellular response to DNA damage stimulus
GO:0007292	female gamete generation
GO:0015979	photosynthesis
GO:0015995	chlorophyll biosynthetic process
GO:0048232	male gamete generation

Cellular Component

GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0097362	MCM8-MCM9 complex

Protein Structure and Domains

PDB ID

InterPro

IPR000523 Magnesium chelatase, ChlI subunit
IPR001208 Mini-chromosome maintenance, DNA-dependent ATPase
IPR003593 AAA+ ATPase domain
IPR011704 ATPase, dynein-related, AAA domain
IPR012340 Nucleic acid-binding, OB-fold
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM

PF01078
PF00493
PF07728

PRINTS

PR01657

PIRSF

SMART

SM00350
SM00382

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt