InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: NUAK1

Summary

InnateDB Protein

IDBP-54707.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

NUAK1

Protein Name

NUAK family, SNF1-like kinase, 1

Synonyms

ARK5;

Species

Homo sapiens

Ensembl Protein

ENSP00000261402

InnateDB Gene

IDBG-54705 (NUAK1)

Protein Structure

UniProt Annotation

Function

Serine/threonine-protein kinase involved in various processes such as cell adhesion, regulation of cell ploidy and senescence, cell proliferation and tumor progression. Phosphorylates ATM, CASP6, LATS1, PPP1R12A and p53/TP53. Acts as a regulator of cellular senescence and cellular ploidy by mediating phosphorylation of 'Ser-464' of LATS1, thereby controlling its stability. Controls cell adhesion by regulating activity of the myosin protein phosphatase 1 (PP1) complex. Acts by mediating phosphorylation of PPP1R12A subunit of myosin PP1: phosphorylated PPP1R12A then interacts with 14-3-3, leading to reduced dephosphorylation of myosin MLC2 by myosin PP1. May be involved in DNA damage response: phosphorylates p53/TP53 at 'Ser-15' and 'Ser- 392' and is recruited to the CDKN1A/WAF1 promoter to participate to transcription activation by p53/TP53. May also act as a tumor malignancy-associated factor by promoting tumor invasion and metastasis under regulation and phosphorylation by AKT1. Suppresses Fas-induced apoptosis by mediating phosphorylation of CASP6, thereby suppressing the activation of the caspase and the subsequent cleavage of CFLAR. {ECO:0000269PubMed:12409306, ECO:0000269PubMed:14976552, ECO:0000269PubMed:15060171, ECO:0000269PubMed:15273717, ECO:0000269PubMed:19927127, ECO:0000269PubMed:20354225, ECO:0000269PubMed:21317932}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:21317932}. Cytoplasm {ECO:0000269PubMed:21317932}.

Disease Associations

Tissue Specificity

Expressed at high levels in heart and brain, and at lower levels in skeletal muscle, kidney, ovary, placenta, lung and liver. Highly up-regulated in colorectal cancer cell lines. {ECO:0000269PubMed:14982852}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 29 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.

Experimentally validated
Total	29 [view]
Protein-Protein	27 [view]
Protein-DNA	1 [view]
Protein-RNA	1 [view]
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	1 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0002039	p53 binding
GO:0004672	protein kinase activity
GO:0004674	protein serine/threonine kinase activity
GO:0004713	protein tyrosine kinase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016772	transferase activity, transferring phosphorus-containing groups
GO:0016773	phosphotransferase activity, alcohol group as acceptor
GO:0046872	metal ion binding

Biological Process

GO:0006468	protein phosphorylation
GO:0006974	cellular response to DNA damage stimulus
GO:0007155	cell adhesion
GO:0008152	metabolic process
GO:0009103	lipopolysaccharide biosynthetic process
GO:0030155	regulation of cell adhesion
GO:0035507	regulation of myosin-light-chain-phosphatase activity
GO:0042127	regulation of cell proliferation
GO:2000772	regulation of cellular senescence

Cellular Component

GO:0005634	nucleus
GO:0005730	nucleolus
GO:0005737	cytoplasm
GO:0015630	microtubule cytoskeleton
GO:0016020	membrane

Protein Structure and Domains

PDB ID

InterPro

IPR000719 Protein kinase domain
IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain
IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain
IPR010440 Lipopolysaccharide kinase
IPR011009 Protein kinase-like domain
IPR020635 Tyrosine-protein kinase, catalytic domain

PFAM

PF00069
PF07714
PF06293

PRINTS

PR00109

PIRSF

SMART

SM00220
SM00219

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt