InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: ASTL

Summary

InnateDB Protein

IDBP-61484.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

ASTL

Protein Name

astacin-like metallo-endopeptidase (M12 family)

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000343674

InnateDB Gene

IDBG-61482 (ASTL)

Protein Structure

UniProt Annotation

Function

Oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization. Plays a role in the polyspermy inhibition. Probably acts as a protease for the post-fertilization cleavage of ZP2. Cleaves the sperm-binding ZP2 at the surface of the zona pellucida after fertilization and cortical granule exocytosis, rendering the zona pellucida unable to support further sperm binding (By similarity). {ECO:0000250}.

Subcellular Localization

Cytoplasm {ECO:0000250}. Cell membrane {ECO:0000250}. Cytoplasmic granule {ECO:0000250}. Cytoplasmic vesicle, secretory vesicle {ECO:0000250}. Note=Secretory granules. Localizes to the peripheral cortical granules of ovulated eggs. Probably exocytosed from cortical granules during post- fertilization. Detected throughout the ooplasm of germinal vesicle stage oocytes in early bilaminar secondary follicles shortly after birth. Detected in the microvillar domain of the oolemma in arrested ovulated secondary oocytes and in the first polar body prior to fertilization. Upon fertilization, detected in the perivitelline space (PVS). Colocalizes with SPACA3 at the microvillar domain of the oolemma and in the perivitelline space (PVS) (By similarity). {ECO:0000250}.

Disease Associations

Tissue Specificity

Expressed in promyelocytic leukemia HL-60 cells, Burkitt's lymphoma Raji cells, and also in some ovarian carcinomas. Not detected in normal tissues. {ECO:0000269PubMed:15087446}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 1 experimentally validated interaction(s) in this database.

Experimentally validated
Total	1 [view]
Protein-Protein	0
Protein-DNA	0
Protein-RNA	0
DNA-DNA	1 [view]
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004222	metalloendopeptidase activity
GO:0005515	protein binding
GO:0008233	peptidase activity
GO:0008237	metallopeptidase activity
GO:0008270	zinc ion binding
GO:0070001	aspartic-type peptidase activity
GO:0070002	glutamic-type peptidase activity

Biological Process

GO:0006508	proteolysis
GO:0007155	cell adhesion
GO:0009566	fertilization
GO:0010954	positive regulation of protein processing
GO:0060468	prevention of polyspermy
GO:2000360	negative regulation of binding of sperm to zona pellucida