InnateDB: Systems Biology of the Innate Immune Response

Bos taurus Protein: AP2S1

Summary

InnateDB Protein

IDBP-692144.2

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

AP2S1

Protein Name

AP-2 complex subunit sigma

Synonyms

Species

Bos taurus

Ensembl Protein

ENSBTAP00000013989

InnateDB Gene

IDBG-639941 (AP2S1)

Protein Structure

UniProt Annotation

Function

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. May also play a role in extracellular calcium homeostasis (By similarity). {ECO:0000250}.

Subcellular Localization

Cell membrane {ECO:0000250}. Membrane, coated pit {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Note=AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV. {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 0 experimentally validated interaction(s) in this database.
They are also associated with 9 interaction(s) predicted by orthology.

Predicted by orthology
Total	9 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0008565	protein transporter activity

Biological Process

GO:0006810	transport
GO:0006886	intracellular protein transport
GO:0006897	endocytosis
GO:0015031	protein transport
GO:0016192	vesicle-mediated transport

Cellular Component

GO:0005886	plasma membrane
GO:0005905	coated pit
GO:0016020	membrane
GO:0030117	membrane coat
GO:0030122	AP-2 adaptor complex

Protein Structure and Domains

PDB ID

InterPro

IPR011012 Longin-like domain
IPR016635 Adaptor protein complex, sigma subunit
IPR022775 AP complex, mu/sigma subunit

PFAM

PF01217

PRINTS

PIRSF

PIRSF015588

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q17QC5

PhosphoSite

PhosphoSite-

TrEMBL

UniProt Splice Variant

Entrez Gene

536553

UniGene

Bt.89386

RefSeq

NP_001069546

HUGO

OMIM

CCDS

HPRD

IMGT

EMBL

BC118437

GenPept

AAI18438