InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Rbm4

Summary

InnateDB Protein

IDBP-704806.3

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Rbm4

Protein Name

Synonyms

4921506I22Rik; AI504630; AI506404; Lark2;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000137345

InnateDB Gene

IDBG-702356 (Rbm4)

Protein Structure

UniProt Annotation

Function

RNA-binding factor involved in multiple aspects of cellular processes like alternative splicing of pre-mRNA and translation regulation. Modulates alternative 5'-splice site and exon selection. Acts as a muscle cell differentiation-promoting factor. Activates exon skipping of the PTB pre-mRNA during muscle cell differentiation. Antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin. Binds to intronic pyrimidine-rich sequence of the TPM1 and MAPT pre-mRNAs. Required for the translational activation of PER1 mRNA in response to circadian clock. Binds directly to the 3'-UTR of the PER1 mRNA. Exerts a suppressive activity on Cap-dependent translation via binding to CU-rich responsive elements within the 3'UTR of mRNAs, a process increased under stress conditions or during myocytes differentiation. Recruits EIF4A1 to stimulate IRES-dependent translation initiation in respons to cellular stress. Associates to internal ribosome entry segment (IRES) in target mRNA species under stress conditions. Plays a role for miRNA-guided RNA cleavage and translation suppression by promoting association of AGO2- containing miRNPs with their cognate target mRNAs. Associates with miRNAs during muscle cell differentiation. Binds preferentially to 5'-CGCGCG[GCA]-3' motif in vitro. {ECO:0000269PubMed:17264215}.

Subcellular Localization

Nucleus {ECO:0000250}. Nucleus, nucleolus {ECO:0000250}. Nucleus speckle {ECO:0000250}. Cytoplasm {ECO:0000250}. Cytoplasmic granule {ECO:0000250}. Note=Undergoes continuous nucleocytoplasmic shuttling. Upon nuclear import colocalizes with SR proteins in nuclear speckles. Arsenite stress- induced phosphorylation increases its subcellular relocalization from the nucleus to the cytoplasm and to cytoplasmic stress granules (SG) via a p38 MAPK signaling pathway. Primarily localized in nucleus and nucleoli under cell growth conditions and accumulated in the cytoplasm and cytoplasm perinuclear granules upon muscle cell differentiation (By similarity). {ECO:0000250}.

Disease Associations

Tissue Specificity

Expressed in the suprachiasmatic nucleus (SCN). Expressed in myocytes; expression gradually increases during muscle cell differentiation (at protein level). Expressed in the suprachiasmatic nucleus (SCN). {ECO:0000269PubMed:17264215, ECO:0000269PubMed:19801630, ECO:0000269PubMed:21518792}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 1 experimentally validated interaction(s) in this database.
They are also associated with 2 interaction(s) predicted by orthology.

Experimentally validated
Total	1 [view]
Protein-Protein	1 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	2 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000166	nucleotide binding
GO:0003676	nucleic acid binding
GO:0003723	RNA binding
GO:0003730	mRNA 3'-UTR binding
GO:0008270	zinc ion binding
GO:0035198	miRNA binding
GO:0036002	pre-mRNA binding
GO:0097157	pre-mRNA intronic binding
GO:0097158	pre-mRNA intronic pyrimidine-rich binding

Biological Process

GO:0000381	regulation of alternative mRNA splicing, via spliceosome
GO:0002190	cap-independent translational initiation
GO:0002192	IRES-dependent translational initiation
GO:0006397	mRNA processing
GO:0008380	RNA splicing
GO:0017148	negative regulation of translation
GO:0030154	cell differentiation
GO:0032055	negative regulation of translation in response to stress
GO:0035278	negative regulation of translation involved in gene silencing by miRNA
GO:0043153	entrainment of circadian clock by photoperiod
GO:0045947	negative regulation of translational initiation
GO:0046685	response to arsenic-containing substance
GO:0046822	regulation of nucleocytoplasmic transport
GO:0051149	positive regulation of muscle cell differentiation
GO:0051403	stress-activated MAPK cascade
GO:0097167	circadian regulation of translation

Cellular Component

GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005730	nucleolus
GO:0005737	cytoplasm
GO:0010494	cytoplasmic stress granule
GO:0016607	nuclear speck
GO:0048471	perinuclear region of cytoplasm