InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: RPTOR

Summary

InnateDB Protein

IDBP-71822.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

RPTOR

Protein Name

regulatory associated protein of MTOR, complex 1

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000307272

InnateDB Gene

IDBG-71820 (RPTOR)

Protein Structure

UniProt Annotation

Function

Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis. {ECO:0000269PubMed:12150925, ECO:0000269PubMed:12150926, ECO:0000269PubMed:23727834}.

Subcellular Localization

Cytoplasm. Lysosome. Cytoplasmic granule. Note=Targeting to lysosomes depends on amino acid availability. In arsenite-stressed cells, accumulates in stress granules when associated with SPAG5 and association with lysosomes is drastically decreased.

Disease Associations

Tissue Specificity

Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. Isoform 3 is widely expressed, with highest levels in nasal mucosa and pituitary and lowest in spleen. {ECO:0000269PubMed:12150925, ECO:0000269PubMed:12408816}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 127 experimentally validated interaction(s) in this database.
They are also associated with 4 interaction(s) predicted by orthology.

Experimentally validated
Total	127 [view]
Protein-Protein	127 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	4 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0001030	RNA polymerase III type 1 promoter DNA binding
GO:0001031	RNA polymerase III type 2 promoter DNA binding
GO:0001032	RNA polymerase III type 3 promoter DNA binding
GO:0001156	TFIIIC-class transcription factor binding
GO:0005488	binding
GO:0005515	protein binding
GO:0019901	protein kinase binding
GO:0032403	protein complex binding
GO:0071889	14-3-3 protein binding

Biological Process

GO:0001938	positive regulation of endothelial cell proliferation
GO:0007050	cell cycle arrest
GO:0008286	insulin receptor signaling pathway
GO:0008361	regulation of cell size
GO:0016049	cell growth
GO:0031669	cellular response to nutrient levels
GO:0031929	TOR signaling
GO:0032008	positive regulation of TOR signaling
GO:0042325	regulation of phosphorylation
GO:0045945	positive regulation of transcription from RNA polymerase III promoter
GO:0071230	cellular response to amino acid stimulus
GO:0071902	positive regulation of protein serine/threonine kinase activity

Cellular Component

GO:0005737	cytoplasm
GO:0005764	lysosome
GO:0005765	lysosomal membrane
GO:0005829	cytosol
GO:0030425	dendrite
GO:0031931	TORC1 complex
GO:0043025	neuronal cell body