InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: TRIM27

Summary

InnateDB Protein

IDBP-72255.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

TRIM27

Protein Name

tripartite motif containing 27

Synonyms

RFP; RNF76;

Species

Homo sapiens

Ensembl Protein

ENSP00000366404

InnateDB Gene

IDBG-72253 (TRIM27)

Protein Structure

UniProt Annotation

Function

E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T- cell activation. Acts as a regulator of retrograde transport: together with MAGEL2, mediates the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASH1, leading to promote endosomal F-actin assembly (PubMed:23452853). Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double- stranded DNA. {ECO:0000269PubMed:10976108, ECO:0000269PubMed:12807881, ECO:0000269PubMed:22128329, ECO:0000269PubMed:23452853}.

Subcellular Localization

Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}. Nucleus, PML body {ECO:0000250}. Early endosome {ECO:0000269PubMed:1437549, ECO:0000269PubMed:23452853, ECO:0000269PubMed:9247190}. Note=Nuclear or cytoplasmic depending on the cell type (By similarity). Colocalized with PML and EIF3S6 in nuclear bodies. Recruited to retromer-containing endosomes via interaction with MAGEL2 (PubMed:23452853). {ECO:0000250, ECO:0000269PubMed:23452853}.

Disease Associations

Thyroid papillary carcinoma (TPC) [MIM:188550]: A common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=The disease is caused by mutations affecting the gene represented in this entry. A chromosomal aberration involving TRIM27/RFP is found in thyroid papillary carcinomas. Translocation t(6;10)(p21.3;q11.2) with RET. The translocation generates TRIM27/RET and delta TRIM27/RET oncogenes.

Tissue Specificity

Expressed in testis namely within the seminiferous tubules. {ECO:0000269PubMed:12445133}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 154 experimentally validated interaction(s) in this database.
They are also associated with 7 interaction(s) predicted by orthology.

Experimentally validated
Total	154 [view]
Protein-Protein	154 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	7 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0004714	transmembrane receptor protein tyrosine kinase activity
GO:0004842	ubiquitin-protein transferase activity
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0016874	ligase activity
GO:0046872	metal ion binding

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0002820	negative regulation of adaptive immune response
GO:0006351	transcription, DNA-templated
GO:0006469	negative regulation of protein kinase activity
GO:0007283	spermatogenesis
GO:0008283	cell proliferation
GO:0018108	peptidyl-tyrosine phosphorylation
GO:0032720	negative regulation of tumor necrosis factor production
GO:0032897	negative regulation of viral transcription
GO:0034314	Arp2/3 complex-mediated actin nucleation
GO:0042147	retrograde transport, endosome to Golgi
GO:0045087	innate immune response (InnateDB)
GO:0045814	negative regulation of gene expression, epigenetic
GO:0051091	positive regulation of sequence-specific DNA binding transcription factor activity
GO:0070206	protein trimerization
GO:0070534	protein K63-linked ubiquitination
GO:0072643	interferon-gamma secretion
GO:0090281	negative regulation of calcium ion import
GO:1900041	negative regulation of interleukin-2 secretion
GO:1902187	negative regulation of viral release from host cell

Cellular Component

GO:0005622	intracellular
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005737	cytoplasm
GO:0005768	endosome
GO:0005769	early endosome
GO:0005887	integral component of plasma membrane
GO:0016020	membrane
GO:0016605	PML body
GO:0030904	retromer complex
GO:0031965	nuclear membrane

Protein Structure and Domains

PDB ID

InterPro

IPR000315 Zinc finger, B-box
IPR001841 Zinc finger, RING-type
IPR001870 B30.2/SPRY domain
IPR003877 SPRY domain
IPR003879 Butyrophylin-like
IPR006574 SPRY-associated
IPR008985 Concanavalin A-like lectin/glucanases superfamily
IPR018957 Zinc finger, C3HC4 RING-type
IPR020457 Zinc finger, B-box, chordata

PFAM

PF00643
PF13639
PF14634
PF00622
PF13765
PF00097

PRINTS

PR01407
PR01406

PIRSF

SMART

SM00336
SM00184
SM00449
SM00589

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt