Version 5.4
Mus musculus Gene: Fen1
Summary
InnateDB Gene IDBG-143512.6
Last Modified 2014-10-13 [Report errors or provide feedback]
Gene Symbol Fen1
Gene Name flap structure specific endonuclease 1
Synonyms AW538437; FEN-1
Species Mus musculus
Ensembl Gene ENSMUSG00000024742
Encoded Proteins
IDBP-143514
flap structure specific endonuclease 1
IDBP-273869
flap structure specific endonuclease 1
IDBP-532496
flap structure specific endonuclease 1
IDBP-538371
flap structure specific endonuclease 1
Protein Structure
Useful resources Stemformatics EHFPI ImmGen
Entrez Gene
Summary This gene does not have any Entrez summary - the following is the summary from its human ortholog ENSG00000168496:
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq, Jul 2008]
The protein encoded by this gene removes 5\' overhanging flaps in DNA repair and processes the 5\' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5\' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq, Jul 2008]
Gene Information
Type Protein coding
Genomic Location Chromosome 19:10199132-10204169
Strand Reverse strand
Band A
Transcripts
ENSMUST00000025651 ENSMUSP00000025651
ENSMUST00000116542 ENSMUSP00000112241
ENSMUST00000156291 ENSMUSP00000117246
ENSMUST00000142241 ENSMUSP00000119221
Interactions
Number of Interactions This gene and/or its encoded proteins are associated with 2 experimentally validated interaction(s) in this database.
They are also associated with 28 interaction(s) predicted by orthology.
Experimentally validated
Total 2 [view]
Protein-Protein 2 [view]
Protein-DNA 0
Protein-RNA 0
DNA-DNA 0
RNA-RNA 0
DNA-RNA 0
Predicted by orthology
Total 28 [view]
Gene Ontology

Molecular Function
Accession GO Term
GO:0000287 magnesium ion binding
GO:0003677 DNA binding
GO:0003824 catalytic activity
GO:0004518 nuclease activity
GO:0004523 RNA-DNA hybrid ribonuclease activity
GO:0004527 exonuclease activity
GO:0005515 protein binding
GO:0008409 5'-3' exonuclease activity
GO:0017108 5'-flap endonuclease activity
GO:0030145 manganese ion binding
GO:0048256 flap endonuclease activity
Biological Process
GO:0000737 DNA catabolic process, endonucleolytic
GO:0006260 DNA replication
GO:0006281 DNA repair
GO:0006284 base-excision repair
GO:0007613 memory
GO:0043137 DNA replication, removal of RNA primer
GO:0090305 nucleic acid phosphodiester bond hydrolysis
GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
Cellular Component
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0005739 mitochondrion
GO:0016020 membrane
Orthologs
Species
Homo sapiens
Bos taurus
Gene ID
Gene Order
ENSG00000168496
View Gene Order
ENSBTAG00000000064
Not yet available
Pathways
NETPATH
REACTOME
KEGG
mmu03030
DNA replication pathway
mmu03410
Base excision repair pathway
mmu03450
Non-homologous end-joining pathway
INOH
PID NCI
Pathway Predictions based on Human Orthology Data
NETPATH
REACTOME
REACT_7999
Removal of the Flap Intermediate from the C-strand pathway
REACT_8027
Processive synthesis on the C-strand of the telomere pathway
REACT_7961
Telomere C-strand (Lagging Strand) Synthesis pathway
REACT_70
Removal of the Flap Intermediate pathway
REACT_1385
Processive synthesis on the lagging strand pathway
REACT_2192
Removal of DNA patch containing abasic residue pathway
REACT_1128
Resolution of AP sites via the multiple-nucleotide patch replacement pathway pathway
REACT_383
DNA Replication pathway
REACT_2014
Synthesis of DNA pathway
REACT_1893
Resolution of Abasic Sites (AP sites) pathway
REACT_899
S Phase pathway
REACT_22172
Chromosome Maintenance pathway
REACT_7970
Telomere Maintenance pathway
REACT_115566
Cell Cycle pathway
REACT_1104
Base Excision Repair pathway
REACT_152
Cell Cycle, Mitotic pathway
REACT_932
DNA strand elongation pathway
REACT_8030
Extension of Telomeres pathway
REACT_312
Lagging Strand Synthesis pathway
REACT_216
DNA Repair pathway
REACT_225014
S Phase pathway
REACT_209654
Extension of Telomeres pathway
REACT_222852
Removal of the Flap Intermediate from the C-strand pathway
REACT_213604
DNA Replication pathway
REACT_221747
Telomere Maintenance pathway
REACT_204980
Resolution of Abasic Sites (AP sites) pathway
REACT_206699
Removal of the Flap Intermediate pathway
REACT_215505
Cell Cycle, Mitotic pathway
REACT_202907
Telomere C-strand (Lagging Strand) Synthesis pathway
REACT_221571
Processive synthesis on the lagging strand pathway
REACT_205725
Processive synthesis on the C-strand of the telomere pathway
REACT_224922
Synthesis of DNA pathway
REACT_214934
Cell Cycle pathway
REACT_204262
DNA Repair pathway
REACT_214081
Lagging Strand Synthesis pathway
REACT_227100
Removal of DNA patch containing abasic residue pathway
REACT_202612
Base Excision Repair pathway
REACT_227796
Chromosome Maintenance pathway
REACT_218453
Resolution of AP sites via the multiple-nucleotide patch replacement pathway pathway
REACT_203054
DNA strand elongation pathway
KEGG
hsa03030
DNA replication pathway
hsa03450
Non-homologous end-joining pathway
hsa03410
Base excision repair pathway
INOH
PID NCI
Cross-References
SwissProt
TrEMBL
UniProt Splice Variant
Entrez Gene
UniGene Mm.2952
RefSeq NM_001271614 NM_001271615 NM_007999 XM_006526661 XM_006526662 XM_006526663 XM_006526664
OMIM
CCDS CCDS29573
HPRD
IMGT
MGI ID
MGI Symbol
EMBL
GenPept
RNA Seq Atlas

If you use InnateDB for your research, please cite the following publication:

Breuer et al., InnateDB: systems biology of innate immunity and beyond - recent updates and continuing curation. Nucl. Acids Res. (2013) 41 (D1) 

InnateDB is being developed jointly by the Brinkman Laboratory (Simon Fraser University, British Columbia, Canada), the Hancock Laboratory (University of British Columbia, Vancouver, British Columbia) and the Lynn EMBL Australia Group (South Australian Health & Medical Research Institute and Flinders University, Adelaide, Australia).

Funding is currently provided by Allergen and EMBL Australia. Previous funding has been provided by Genome Canada, the Foundation for the National Institutes of Health through the Grand Challenges in Global Health initiative and by Teagasc. InnateDB curated interactions are licensed under the Design Science License. All other data is licensed under the terms of the originating database. Contact: innatedb-mail@sfu.ca