InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Gene: ASAP2

Summary

InnateDB Gene

IDBG-27381.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

ASAP2

Gene Name

ArfGAP with SH3 domain, ankyrin repeat and PH domain 2

Synonyms

AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha; SHAG1

Species

Homo sapiens

Ensembl Gene

ENSG00000151693

Encoded Proteins

IDBP-27383

ArfGAP with SH3 domain, ankyrin repeat and PH domain 2

IDBP-27385

ArfGAP with SH3 domain, ankyrin repeat and PH domain 2

Protein Structure

Useful resources

Stemformatics EHFPI ImmGen

Entrez Gene

Summary

This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Gene Information

Type

Protein coding

Genomic Location

Chromosome 2:9206765-9405683

Strand

Forward strand

Band

p25.1

Transcripts

ENST00000281419	ENSP00000281419
ENST00000315273	ENSP00000316404
ENST00000471687
ENST00000491413
ENST00000484590

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 21 experimentally validated interaction(s) in this database.
They are also associated with 3 interaction(s) predicted by orthology.

Experimentally validated
Total	21 [view]
Protein-Protein	20 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	3 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005515	protein binding
GO:0008047	enzyme activator activity
GO:0008060	ARF GTPase activator activity
GO:0008270	zinc ion binding

Biological Process

GO:0032312	regulation of ARF GTPase activity
GO:0043085	positive regulation of catalytic activity
GO:0043547	positive regulation of GTPase activity

Cellular Component

GO:0005886	plasma membrane
GO:0032580	Golgi cisterna membrane

Orthologs

Species

Mus musculus

Bos taurus

Gene ID

Gene Order

ENSMUSG00000052632

View Gene Order

ENSBTAG00000002329

Not yet available

Pathways

NETPATH

REACTOME

KEGG

hsa04666

Fc gamma R-mediated phagocytosis pathway

hsa04144

Endocytosis pathway

INOH

PID NCI

arf6_traffickingpathway

Arf6 trafficking events

Cross-References

SwissProt

TrEMBL

UniProt Splice Variant

Entrez Gene

UniGene

Hs.555902 Hs.695627

RefSeq

NM_001135191 NM_003887 XM_006711898 XM_006711899 XM_006711900 XM_006711901 XM_006711902

HUGO

OMIM

CCDS

CCDS1661 CCDS46224

HPRD

04820

IMGT

EMBL

GenPept

RNA Seq Atlas