InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Gene: DFFA

Summary

InnateDB Gene

IDBG-89047.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

DFFA

Gene Name

DNA fragmentation factor, 45kDa, alpha polypeptide

Synonyms

DFF-45; DFF1; ICAD

Species

Homo sapiens

Ensembl Gene

ENSG00000160049

Encoded Proteins

IDBP-89049

DNA fragmentation factor, 45kDa, alpha polypeptide

IDBP-89051

DNA fragmentation factor, 45kDa, alpha polypeptide

IDBP-722534

Protein Structure

Useful resources

Stemformatics EHFPI ImmGen

Entrez Gene

Summary

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Gene Information

Type

Protein coding

Genomic Location

Chromosome 1:10456522-10472526

Strand

Reverse strand

Band

p36.22

Transcripts

ENST00000377038	ENSP00000366237
ENST00000377036	ENSP00000366235
ENST00000476658	ENSP00000468395

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 47 experimentally validated interaction(s) in this database.
They are also associated with 2 interaction(s) predicted by orthology.

Experimentally validated
Total	47 [view]
Protein-Protein	46 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	1 [view]

Predicted by orthology
Total	2 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005515	protein binding

Biological Process

GO:0006309	apoptotic DNA fragmentation
GO:0006915	apoptotic process
GO:0006921	cellular component disassembly involved in execution phase of apoptosis
GO:0007165	signal transduction
GO:0043065	positive regulation of apoptotic process
GO:0070242	thymocyte apoptotic process
GO:1900118	negative regulation of execution phase of apoptosis
GO:1902511	negative regulation of apoptotic DNA fragmentation

Cellular Component

GO:0000790	nuclear chromatin
GO:0005622	intracellular
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005737	cytoplasm
GO:0005829	cytosol

Orthologs

Species

Mus musculus

Bos taurus

Gene ID

Gene Order

ENSMUSG00000028974

View Gene Order

ENSBTAG00000013535

Not yet available

Pathways

NETPATH

REACTOME

REACT_13462

Activation of DNA fragmentation factor pathway

REACT_995

Apoptotic execution phase pathway

REACT_1213

Apoptosis induced DNA fragmentation pathway

REACT_578

Apoptosis pathway

KEGG

hsa04210

Apoptosis pathway

INOH

PID NCI

caspase_pathway

Caspase Cascade in Apoptosis

Cross-References

SwissProt

O00273

TrEMBL

A0A024R4H5 K7ERT1

UniProt Splice Variant

Entrez Gene

1676

UniGene

Hs.484782 Hs.48617 Hs.603104

RefSeq

NM_004401 NM_213566

HUGO

HGNC:2772

OMIM

601882

CCDS

CCDS118 CCDS119

HPRD

IMGT

EMBL

AF087573 AF103799 AK313317 AL354956 BC000037 BC007112 BC007721 BT006980 CH471130 U91985

GenPept

AAC51249 AAD32953 AAF02419 AAH00037 AAH07112 AAH07721 AAP35626 BAG36122 CAI19196 CAI19197 EAW71656 EAW71657 EAW71658

RNA Seq Atlas

1676