Version 5.4
Homo sapiens Gene: DFFB
Summary
InnateDB Gene IDBG-86952.6
Last Modified 2014-10-13 [Report errors or provide feedback]
Gene Symbol DFFB
Gene Name DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
Synonyms CAD; CPAN; DFF-40; DFF2; DFF40
Species Homo sapiens
Ensembl Gene ENSG00000169598
Encoded Proteins
IDBP-86956
DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
IDBP-86958
DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
IDBP-86960
DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
IDBP-86962
DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
IDBP-86964
DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
IDBP-585958
DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
IDBP-722152
IDBP-722496
IDBP-721797
IDBP-721955
Protein Structure
Useful resources Stemformatics EHFPI ImmGen
Entrez Gene
Summary Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined. [provided by RefSeq, Jul 2008]
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined. [provided by RefSeq, Sep 2013]
Gene Information
Type Protein coding
Genomic Location Chromosome 1:3857267-3885429
Strand Forward strand
Band p36.32
Transcripts
ENST00000378209 ENSP00000367454
ENST00000338895 ENSP00000339524
ENST00000341385 ENSP00000345906
ENST00000339350 ENSP00000343218
ENST00000378206 ENSP00000367448
ENST00000491998 ENSP00000436775
ENST00000468793 ENSP00000463688
ENST00000477548 ENSP00000462175
ENST00000475969 ENSP00000463704
ENST00000481945
ENST00000461150 ENSP00000462770
Interactions
Number of Interactions This gene and/or its encoded proteins are associated with 20 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.
Experimentally validated
Total 20 [view]
Protein-Protein 20 [view]
Protein-DNA 0
Protein-RNA 0
DNA-DNA 0
RNA-RNA 0
DNA-RNA 0
Predicted by orthology
Total 1 [view]
Gene Ontology

Molecular Function
Accession GO Term
GO:0003677 DNA binding
GO:0004518 nuclease activity
GO:0004536 deoxyribonuclease activity
GO:0005515 protein binding
GO:0016787 hydrolase activity
GO:0019899 enzyme binding
Biological Process
GO:0006309 apoptotic DNA fragmentation
GO:0006915 apoptotic process
GO:0006921 cellular component disassembly involved in execution phase of apoptosis
GO:0007420 brain development
GO:0030263 apoptotic chromosome condensation
Cellular Component
GO:0000790 nuclear chromatin
GO:0005622 intracellular
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005829 cytosol
Orthologs
Species
Mus musculus
Bos taurus
Gene ID
Gene Order
ENSMUSG00000029027
View Gene Order
ENSBTAG00000020015
Not yet available
Pathways
NETPATH
REACTOME
REACT_13462
Activation of DNA fragmentation factor pathway
REACT_995
Apoptotic execution phase pathway
REACT_1213
Apoptosis induced DNA fragmentation pathway
REACT_578
Apoptosis pathway
KEGG
hsa04210
Apoptosis pathway
INOH
PID NCI
caspase_pathway
Caspase Cascade in Apoptosis
Cross-References
SwissProt
TrEMBL
UniProt Splice Variant
Entrez Gene
UniGene Hs.133089
RefSeq NM_001282669 NM_004402
HUGO
OMIM
CCDS CCDS52 CCDS72693
HPRD 03532
IMGT
EMBL
GenPept
RNA Seq Atlas

If you use InnateDB for your research, please cite the following publication:

Breuer et al., InnateDB: systems biology of innate immunity and beyond - recent updates and continuing curation. Nucl. Acids Res. (2013) 41 (D1) 

InnateDB is being developed jointly by the Brinkman Laboratory (Simon Fraser University, British Columbia, Canada), the Hancock Laboratory (University of British Columbia, Vancouver, British Columbia) and the Lynn EMBL Australia Group (South Australian Health & Medical Research Institute and Flinders University, Adelaide, Australia).

Funding is currently provided by Allergen and EMBL Australia. Previous funding has been provided by Genome Canada, the Foundation for the National Institutes of Health through the Grand Challenges in Global Health initiative and by Teagasc. InnateDB curated interactions are licensed under the Design Science License. All other data is licensed under the terms of the originating database. Contact: innatedb-mail@sfu.ca