InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Gene: NCF4

Summary

InnateDB Gene

IDBG-6112.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

NCF4

Gene Name

neutrophil cytosolic factor 4, 40kDa

Synonyms

NCF; P40PHOX; SH3PXD4

Species

Homo sapiens

Ensembl Gene

ENSG00000100365

Encoded Proteins

IDBP-6114

neutrophil cytosolic factor 4, 40kDa

IDBP-235973

neutrophil cytosolic factor 4, 40kDa

IDBP-374993

neutrophil cytosolic factor 4, 40kDa

IDBP-374997

neutrophil cytosolic factor 4, 40kDa

Protein Structure

Useful resources

Stemformatics EHFPI ImmGen

Entrez Gene

Summary

The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Gene Information

Type

Protein coding

Genomic Location

Chromosome 22:36860988-36878015

Strand

Forward strand

Band

q12.3

Transcripts

ENST00000248899	ENSP00000248899
ENST00000397147	ENSP00000380334
ENST00000447071	ENSP00000414958
ENST00000415063	ENSP00000408619

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 13 experimentally validated interaction(s) in this database.
They are also associated with 4 interaction(s) predicted by orthology.

Experimentally validated
Total	13 [view]
Protein-Protein	13 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	4 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005515	protein binding
GO:0016176	superoxide-generating NADPH oxidase activator activity
GO:0032266	phosphatidylinositol-3-phosphate binding
GO:0035091	phosphatidylinositol binding
GO:0046983	protein dimerization activity

Biological Process

GO:0002474	antigen processing and presentation of peptide antigen via MHC class I
GO:0002479	antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
GO:0006955	immune response
GO:0042590	antigen processing and presentation of exogenous peptide antigen via MHC class I
GO:0043085	positive regulation of catalytic activity
GO:0051701	interaction with host
GO:0055114	oxidation-reduction process
GO:0090382	phagosome maturation