Homo sapiens Gene: NCF4
Summary
InnateDB Gene IDBG-6112.6
Last Modified 2014-10-13 [Report errors or provide feedback]
Gene Symbol NCF4
Gene Name neutrophil cytosolic factor 4, 40kDa
Synonyms NCF; P40PHOX; SH3PXD4;
Species Homo sapiens
Ensembl Gene ENSG00000100365
Encoded Proteins
neutrophil cytosolic factor 4, 40kDa
neutrophil cytosolic factor 4, 40kDa
neutrophil cytosolic factor 4, 40kDa
neutrophil cytosolic factor 4, 40kDa
Protein Structure
Useful resources Stemformatics EHFPI ImmGen
Entrez Gene
Summary The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Gene Information
Type Protein coding
Genomic Location Chromosome 22:36860988-36878015
Strand Forward strand
Band q12.3
Transcripts
ENST00000248899 ENSP00000248899
ENST00000397147 ENSP00000380334
ENST00000447071 ENSP00000414958
ENST00000415063 ENSP00000408619
Interactions
Number of Interactions This gene and/or its encoded proteins are associated with 13 experimentally validated interaction(s) in this database.
They are also associated with 4 interaction(s) predicted by orthology.
Experimentally validated
Total 13 [view]
Protein-Protein 13 [view]
Protein-DNA 0
Protein-RNA 0
DNA-DNA 0
RNA-RNA 0
DNA-RNA 0
Predicted by orthology
Total 4 [view]
Gene Ontology

Molecular Function
Accession GO Term
GO:0005515 protein binding
GO:0016176 superoxide-generating NADPH oxidase activator activity
GO:0032266 phosphatidylinositol-3-phosphate binding
GO:0035091 phosphatidylinositol binding
GO:0046983 protein dimerization activity
Biological Process
GO:0002474 antigen processing and presentation of peptide antigen via MHC class I
GO:0002479 antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
GO:0006955 immune response
GO:0042590 antigen processing and presentation of exogenous peptide antigen via MHC class I
GO:0043085 positive regulation of catalytic activity
GO:0051701 interaction with host
GO:0055114 oxidation-reduction process
GO:0090382 phagosome maturation
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0010008 endosome membrane
GO:0016020 membrane
GO:0032010 phagolysosome
GO:0043020 NADPH oxidase complex
Orthologs
Species
Mus musculus
Bos taurus
Gene ID
Gene Order
Method
Confidence
Comments
SSD Ortholog
Ortholog supports species divergence
Not yet available
SSD Ortholog
Ortholog supports species divergence
Pathways
NETPATH
REACTOME
Signaling by VEGF pathway
Cross-presentation of particulate exogenous antigens (phagosomes) pathway
Signal Transduction pathway
Antigen processing-Cross presentation pathway
Phagosomal maturation (early endosomal stage) pathway
Adaptive Immune System pathway
Immune System pathway
VEGFA-VEGFR2 Pathway pathway
Class I MHC mediated antigen processing & presentation pathway
Latent infection of Homo sapiens with Mycobacterium tuberculosis pathway
Disease pathway
KEGG
Phagosome pathway
Osteoclast differentiation pathway
Leukocyte transendothelial migration pathway
Leishmaniasis pathway
INOH
PID BIOCARTA
PID NCI
Cross-References
SwissProt Q15080
TrEMBL B0QY04
UniProt Splice Variant
Entrez Gene 4689
UniGene
RefSeq NM_000631 NM_013416
HUGO HGNC:7662
OMIM 601488
CCDS CCDS13934 CCDS13935
HPRD
IMGT
EMBL AB025219 AB025220 AK290924 AL008637 BC002798 BT007346 CR456528 DQ314880 U50720 U50721 U50722 U50723 U50724 U50725 U50726 U50727 U50728 U50729 X77094
GenPept AAB39970 AAH02798 AAP36010 ABC40739 BAA89791 BAA89792 BAF83613 CAA15486 CAA54372 CAG30414 CAQ10741
RNA Seq Atlas 4689